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KMID : 0894520080120030251
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Development & Reproduction
2008 Volume.12 No. 3 p.251 ~ p.259
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Effect of Prepubertal Exposure to Di(2-ethylhexyl)phthalate on the Maturation of Rat Seminal Vesicles and Prostate Glands
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Heo Hyun-Jin
Lee Won-Yong
Yoon Yong-Dal
Choi Don-Chan
Lee Sung-Ho
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Abstract
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The plasticizer di(2-ethylhexyl)phthalate(DEHP) is one of the most well known endocrine disrupting chemicals (EDCs) because of its strong anti-androgenic effects on the reproductive and developmental process in male rodents and human. The present study was performed to examine whether prepubertal exposure to DEHP can make any alteration during the maturation of accessory sex organs in male rats. As a result, there was no significant change in body weights, serum T levels and tissue weights except of seminal vesicle and ventral prostate in DEHP-treated animals compared to vehicle-treated ones. The seminal vesicle weights in high-dose group (200 ㎎/㎏) were significantly lower than those from the control group (p＜0.05), and ventral prostate weights were significantly lower than those from the control group (p＜0.05) in both low-dose (20 ㎎/㎏) and high-dose group. Histological studies revealed that the seminal vesicles from DEHP-treated groups showed reduced areas of mucosal folds. Pseudostratified columnar epithelia were observed in the ventral prostates of DEHP-treated samples while cuboidal epithelia were found in the control group. The transcriptional activities of ER-α in seminal vesicle from high-dose group (p＜0.05) were significantly higher than those from the control group, and ER-β expression was significantly decreased in low-dose group (p＜0.05) compared to the control. In ventral prostate, ER-β mRNA levels from low-dose group (p＜0.05) were significantly lower than those from the control group, and significantly increased in high-dose group (p＜0.01). AR expressions, however, were not significantly different in all experimental groups of both seminal vesicle and ventral prostate. In conclusion, the present study demonstrated that (ⅰ) adverse effect (s) of DEHP on sexual maturation during prepubertal period could be limited, (ⅱ) seminal vesicle and prostate gland were sensitive targets to DEHP in prepubertal rats and (ⅲ) the deleterious effects of DEHP might be mediated through ER-associated mechanism.
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KEYWORD
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Prepubertal, DEHP, Seminal vesicle, Prostate gland, Estrogen receptor
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